Tg mbp:mcherry-caax
Webengineered_region ( SO:0000804) plasmid ( SO:0000155) Children: promoter_trap_construct ( SO:0001478) engineered_episome ( SO:0000779) enhancer_trap_construct ( SO:0001479) gene_trap_construct ( … WebTg (mbp:mCherry-CAAX) ( 1) Type engineered_plasmid Regulatory Regions mbpa ( 1) Coding Sequences CAAX ( 1) mCherry ( 1) [Em λ] [Ex λ] 610 (red) 587 (red) …
Tg mbp:mcherry-caax
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WebThe red-fluorescing tg(sox10:mRFP) and tg(mbp:mCherry) lines were used to visualise oligodendrocyte structure early and late in development, respectively, and the green … WebINVESTIGATION Whole Genome Sequencing-Based Mapping and Candidate Identification of Mutations from Fixed Zebrafish Tissue Nicholas E. Sanchez,*, 1Breanne L. Harty,*, ,2 Thomas O’Reilly-Pol,† Sarah D. Ackerman,*,3 Amy L. Herbert,* Melanie Holmgren,* Stephen L. Johnson,† Ryan S. Gray,*,4 and Kelly R. Monk*,2 5 *Department of Developmental …
WebAnalysis of Tg(spx1:mCherry-CAAX); Tg(chx10:gfp) larval hindbrain revealed that spx- 1:mCherry + cells did not colocalise with medially located chx10:GFP ... View in full-text … Web5 May 2016 · The transgenic reporter Tg(mbp:mCherry-CAAX) was generated as part of this investigation. Generation of Fluorescent Fusion Proteins to Visualize Myelination along Single Axons. Full-length cntn1a cDNA was amplified by RT-PCR from 3 dpf total RNA using primers 1 (EcoRI-Kozak-cntn1a-F, ...
WebPlasmid PhyB-mCherry-CAAX from Dr. Chao Tang's lab contains the insert PhyB and is published in Mol Biol Cell. 2013 Aug;24(15):2419-30. doi: 10.1091/mbc.E13-03-0126. Epub … Web24 Jun 2013 · (D) Lateral views of a single axon labeled by cntn1b:mCherry and myelin sheaths labeled with Tg(mbp:EGFP-CAAX) at 4 dpf (top) and 8 dpf (bottom) show that the deposition of new myelin sheaths ...
WebDownload scientific diagram mbp + membrane changes are observed following exposure to lysolecithin. All images are lateral views of the spinal cord with anterior to the left and …
Web25 May 2024 · The Tg (mbp:mCherry-NTR) and Tg (mbp:nls-GFP) lines used were generated as part of this study. Generation of the mbp:mCherry-NTR and mbp:nls-GFP transgenic lines We used the tol2kit [ 63] to construct the plasmids required create the Tg (mbp:mCherry-NTR) and Tg (mbp:nls-GFP lines. ireland vacations by railWeb24 Jun 2013 · In order to test this directly, we analyzed individual axons in wild-type and Tg (mbp:ca-Fyn) animals and found that individual myelinated axons in Tg (mbp:ca-Fyn) were covered by about 69% more myelin along their length compared to controls by 5 dpf (wild-type 35% ± 20%, 13 axons in nine animals; Tg (mbp:ca-Fyn) 59% ± 25, ten axons in ten … orderby in created_atWeb4 May 2024 · The following lines were used in this study: Tg(mbp:EGFP-CAAX) (Almeida et al., 2011), Tg(cntn1b:mCherry) (Czopka et al., 2013), and Tg(claudinK:Gal4) (Münzel et al., 2012). The ue58 allele was identified due to its striking disruption of mbp:EGFP-CAAX along the pLLn during the forward genetic screen, underpinning this study (described in Kegel et … ireland vacations with castle staysWebOur analyses of myelination using Tg(mbp:EGFP-CAAX) indicated that despite the extensive myelination of cell bodies, ... (A and B) Confocal images of single oligodendrocytes labeled mosaically using mbp:mCherry-CAAX in wildtype (A) and caspr sa12772 mutants at 5 dpf. Scale bar, 10 μm. orderby in x++WebARTICLE Pro-inflammatory activation following demyelination is required for myelin clearance and oligodendrogenesis Maria Inˆes Cunha 1,2,3*, Minhui Su1,2 ... ireland vacations for couplesWeb5 Apr 2024 · We previously developed expression plasmids encoding GFP-binding peptide (or nanobody) fused to APEX, which allows EM localisation of GFP-tagged proteins in vivo. Here, we have generated conditionally stable GFP- and … ireland vacations quincy maWebTg(sox10(7.2):mrfp) Type engineered_plasmid Regulatory Regions. sox10 ; Coding Sequences. mRFP ; Contains Sequences None Note None. Plasmid Map . None. Genomic Features . That Utilize Tg(sox10:mRFP) Construct . Genomic Feature Affected Genomic Regions vu234Tg: Transgenics . That Utilize Tg(sox10:mRFP) Construct . ireland vacation packing list