Webb1ユニットは、全反応容量50 μl中、37℃で1時間において、1 μgのλ DNA (dam-/HindIII digest)を消化するために必要な酵素量として定義される。 反応条件: 1X CutSmart Buffer. 37℃でインキュベーション。 1X CutSmart Buffer 組成: ・50 mM Potassium Acetate ・20 mM Tris-acetate Webbnco1 rpob hindiii ( New England Biolabs ) New England Biolabs is a verified supplier New England Biolabs manufactures this product About News Press Release Team Advisors Partners Contact Bioz Stars Bioz vStars 86 Buy from Supplier Structured Review New England Biolabs nco1 rpob hindiii
HindIII NEB
WebbThe HindIII digest of lambda DNA ( c I857 ind 1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1). The approximate mass of DNA in each of the bands is provided (assuming a 1.0 μg load) for approximating the mass of DNA in comparably intense samples of similar size. WebbNon-Specific DNase Activity (16 Hour) - A 50 µl reaction in NEBuffer 2 containing 1 µg of Lambda-HindIII DNA and a minimum of 15 units of MspI Methyltransferase incubated for 16 hours at 37ºC results in a DNA pattern free of detectable nuclease degradation as ... New England Biolabs Product Specification. extra long strap crossbody
New England Biolabs (UK) Ltd - HindIII-HF®
WebbNew England Biolabs Certificate of Analysis M0261L / Lot: 10062089 Page 1 of 2 Product Name: Therminator™ DNA Polymerase Catalog Number: M0261L Concentration: 2,000 U/ml Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 75°C. Packaging Lot Number ... Webb17 juni 2024 · Forward primer and reverse primer were provided by Evrogen RU, AO. DNA isolated from liver cancer cells (originating from the HepG2 cell line; cat no. 85011430; MilliporeSigma) was used for amplification of the mutant C228T fragment. High-Fidelity DNA Polymerase (New England BioLabs Inc., Ipswich, MA, USA) was used to amplify … WebbHindIII-HF ®. High-Fidelity (HF ®) restriction enzymes have the same specificity as native enzymes, but have been engineered for significantly reduced star activity and … extra long straight edge