Ctd110.6 antibody
WebOct 11, 2013 · For immunodetection, CTD110.6 antibody was used because it is widely used for the detection of O-GlcNAcylated proteins. Compared to the α-DG-Fc isolated from the control transfectants, immunoreactivity of α-DG-Fc towards CTD110.6 was elevated upon GTDC2 overexpression (Fig. 1A). WebO-GlcNAc antibody (mAb) (Clone CTD110.6) for use in Immunoprecipitation (IP) ... This antibody recognizes single O-GlcNAc residues in b-O-glycosidic linkage to serine and …
Ctd110.6 antibody
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WebApr 19, 2011 · Results. When T24 human bladder cancer cells were subjected to glucose deprivation, the expression of proteins detected by the O-GlcNAc specific antibody … WebJul 23, 2024 · O-GlcNAc Antibody (CTD110.6) is a mouse monoclonal IgM κ O-GlcNAc antibody, cited in 62 publications, provided at 200 µg/ml; raised against O-GlcNAc …
WebClone CTD110.6 specifically detected serine and threonine residues with beta-O-linked GlcNAc, but not alpha-O-linked GlcNAc. The presence of GlcNAc, but not GalNAc, abolished the detection of the target modification. Clone CTD110.6 did not recognize peptides with unmodified serine and threonine residues (Comer, F.I., et al. (2001). WebFormulation. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA. Preparation. The antibody was purified by chromatography and conjugated with …
WebJun 15, 2001 · The antibody reacts with a large number of proteins from cytoplasmic and nuclear extracts and readily detects in vivo changes in O-GlcNAc modification. These … WebCTD110.6 : Host: Mouse : Isotype: IgM : Immunogen: Synthetic peptide containing serine-O-GlcNAc. Species reactivity: Species independent : Specificity: Recognizes Ser-O-GlcNAc and Thr-O-GlcNAc in eukaryotes. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Crossreactivity:
WebApr 24, 2024 · Among these antibodies, CTD110.6 and RL2 are the most widely used. However, due to a certain degree of amino acid sequence dependency, these antibodies cannot detect all proteins with O-GlcNAcylation. Additionally, a few antibodies for site-specific protein O-GlcNAcylation have been reported, ...
WebThe β-O-linked N-acetylglucosamine (O-GlcNAc) antibody recognizes glycosylation, a post-translational modification, which occurs on serine and threonine residues of proteins in. Ximbio. Find reagents. ... Anti-O … how many difficulties in doom eternalWebApr 18, 2014 · We show that CTD110.6, #3, and #10 antibodies can be used to detect cell surface glycoproteins bearing O-GlcNAc. Cell surface glycoproteins recognized by … how many differentials in awdhttp://bb-china.net/search.html?brand=42&pcategory=0&classd=0&use=0&kw=&promotions=2&inventory=2&category=17&page=21 high thigh shortsWebMonoclonal Anti-β-O-Linked N-Acetylglucosamine (mouse IgM isotype) is derived from the hybridoma CTD110.6 produced by the fusion of mouse myeloma cells (P3X63-Ag8.653 cells) and cells from BALB/c mice immunized with a synthetic peptide YSPTS(O-GlcNAc)PSK conjugated to KLH.This antibody recognizes O-GlcNAc in β-O-glycosidic … how many different xboxes are thereWebTime course studies by western blot with the CTD110.6 antibody demonstrated that i.v. injection in rat at 50 mg/kg increased global protein O-GlcNAcylation in total brain homogenates, starting at 1 h and peaking at 10 h and decreased pS396 tau/Tau-5 starting at 1 h, peaking at 4 h, and restoring to normal at 16 h (Yuzwa et al., 2008). how many dig worlds are thereWebJan 1, 2015 · The specificity of the CTD110.6 antibody immunoreactivity was confirmed by a hapten inhibition test using 0.1 m GlcNAc. B, the levels of O-GlcNAc modification on N1EGF1–36-MycHis quantitated by densitometric analyses of immunoblots represented in A (n = 3; results are shown as mean ± S.D.; *, p < 0.01; **, p < 0.05; unpaired Student's t … high thigh socks sims 4 cchigh things kjv meaning